Improved Method for the Detection of Highly Polar Pesticides and Their Main Metabolites in Foods of Animal Origin: Method Validation and Application to Monitoring Programme

The application of polar pesticides in agricultural production has been great interest due to their low costs and high effectiveness. For this reason, the possibility transfer foods animal origin is concern for human health. manuscript describes implementation validation an analytical method detect pesticides, at regulatory levels, three origin, including bovine fat, chicken eggs, cow milk. was fully validated glyphosate, glufosinate, respective metabolites above-mentioned obtaining fit-for-purpose sensitivity, recoveries (76–119%), repeatability (≤20%), within-laboratory reproducibility experimental measurement uncertainty less than 50% as required by SANTE/11312/2021 criteria. Given satisfactory results, applicability additional molecules belonging same category (AMPA, cyanuric acid, ethephon, fosetyl aluminum, HEPA, maleic hydrazide, N-acetyl-glyphosate) also evaluated order meet possible future requests. Finally, implemented applied analyse samples over period March 2021 August 2022 from two Italian regions (Umbria Marche) within national monitoring programme. In agreement with previously available data, none analysed showed presence glyphosate glufosinate levels above legal limit.